Catalog # Product Description | CN0875 This anti-GAPDH antibody is developed using a peptide derived from human GAPDH protein. |
| Western blot analysis of Jurkat cell lysate with CN0875 |
Species | Rabbit |
Formulation | 500μg/mL in PBS with 50% glycerol |
Immunogen | C-terminal peptide derived from human GAPDH conjugated to KLH |
Purification | Antigen-specific affinity chromatography |
Specificity | The antibody recognizes the 37 kDa GADPH protein as a single band. |
Applications | ELISA (1:2500); WB (1:1000) |
Scientific Description | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. The GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells. For this reason, GAPDH is commonly used by biological researchers as a loading control for Western blotting. |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is one year from date of shipping if properly stored. |
Product Specific References | 1. Gene. 2012. 498(1): 68-74. doi:10.1016/j.gene.2012.01.022 2. Tumor Biol. 2013. 34(3): 1807-1812. doi: 10.1007/s13277-013-0720-4. 3. Tumor Biol. 2013. 34(4): 2397-2401. doi: 10.1007/s13277-013-0789-9.
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