Human IgG Antibody, Agarose
Catalog# Product Description | ICP9715 Affinity purified goat anti-human IgG antibodies were developed using purified human IgG as the immunogen. The antibodies are covalently linked to agarose beads and useful for isolation and purification of human immunoglobulin G. |
| Elution profile of human IgG from affinity chromatography based on the anti-human IgG agarose. i. Serum loading: 250 μL, diluted with distilled water to 1 mL ii. Anti-human IgG Agarose: 2 mL iii. Single Pass: 10 min iv. IgG Binding: >4 mg/mL; remove all the IgG from 250 μL of human serum |
SDS Profile: MW markers (1); Human IgG Std (2); Human serum albumin Std (3); Elution fraction 1-5 (3, 4, 5, 6, 7); Original human serum (8) and human serum flow through (9). |
Species | Goat |
Formulation | 1 mL in 2 mL slurry of 50% glycerol with 0.01% NaN3 |
Immunogen | Pure human IgG (whole molecule) |
Purification | Affinity purified with human IgG immobilized on agarose and absorbed with BSA and mouse antibodies. |
Specificity | Human IgG; does not cross react with human serum albumin or mouse IgG |
Antibody Immoblilzed | >30 mg/mL antigen-specific affinity-purified anti-human IgG |
Binding Capacity | Approximately 5 mg human IgG/mL |
Applications | Human IgG removal, isolation and purification |
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly. |
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