产品说明

应用

GO6983已作为蛋白激酶C(PKC)抑制剂，用以研究其对核输入的影响。它还用作Fc培养基中的补充剂，以在丝裂霉素C处理过的小鼠胚胎成纤维细胞(MEF)上培养小鼠胚胎干(mES)细胞。

包装

1 mg in glass bottle
500 μg in glass bottle

生化/生理作用

蛋白激酶C(PKC)抑制剂选择性抑制几种 PKC 同工酶(PKCα和PKCβ的IC₅₀=7nM；PKCγ 为6 nM；PKCδ 为10 nM；PKCζ 为60nM)，但不抑制PKCμ(IC₅₀=20 mM)。

基本信息

产品性质

安全信息

产品说明

一般描述

A potent, cell-permeable, reversible, and ATP-competitive inhibitor of protein kinase C (PKC) that inhibits several PKC isozymes (IC₅₀ = 7 nM for PKC_α and PKC_β; 6 nM for PKC_γ; 10 nM for PKC_δ; and 60 nM for PKC_ζ). Gö 6983 does not effectively inhibit PKC_µ (IC₅₀ = 20 µM) and can thus be used to differentiate PKC_µ from other PKC isozymes.
A potent, cell-permeable, inhibitor of protein kinase C (PKC) that has been shown to selectively inhibit several PKC isozymes (IC₅₀ = 7 nM for PKC_α and PKC_β; 6 nM for PKC_γ; 10 nM for PKC_δ; 60 nM for PKC_ζ). Gö 6983 does not effectively inhibit PKC_µ (IC₅₀ = 20 µM) and can thus be used to differentiate PKC_µ from other PKC isozymes.

包装

500 μg in Plastic ampoule

生化/生理作用

Cell permeable: yes
Primary Target
PKCα and PKCβ
Product competes with ATP.
Reversible: yes
Target IC₅₀: 7 nM for PKCα and PKCβ; 6 nM for PKCγ; 10 nM for PKCδ; and 60 nM for PKCζ

警告

Toxicity: Standard Handling (A)

其他说明

Wang, D., et al. 1998. J. Biol. Chem. 273, 33027.
Stempka, L., et al. 1997. J. Biol. Chem. 272, 6805.
Gschwendt, M., et al. 1996. FEBS Lett. 392, 77.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

产品性质

安全信息