应用
微生物体系的APEX探针底物
特点和优势
分子体积更小（MW: 217.27）； 细胞壁和细胞膜穿透性能更好，水溶液中溶解度更高； 良好空间特异性和更高覆盖度，使APEX标记效率提高一个数量级（图1）。
![图3 APEX2-Mediated Alk-Ph Labeling of the Mitochondrial Transcriptome<sup>[6]</sup>. (A) Scheme of RNA labeling in yeast. (B) Real-time-PCR analysis of mitochondrial RNA enrichment elative to the cytoplasmic RNA markers, ACT1 and THD3.](https://shop.jkchemical.com/res/0-5523.jpg)
图3 APEX2-Mediated Alk-Ph Labeling of the Mitochondrial Transcriptome[6]. (A) Scheme of RNA labeling in yeast. (B) Real-time-PCR analysis of mitochondrial RNA enrichment elative to the cytoplasmic RNA markers, ACT1 and THD3.
参考文献
Li, Y., Tian, C., Liu, K., Zhou, Y., Yang, J., and Zou, P. (2020). A Clickable APEX Probe for Proximity-Dependent Proteomic Profiling in Yeast. Cell Chem Biol 27, 858-865.e858.
相关阅读
邹鹏研究员科研成果 | 应用于微生物体系的APEX探针底物——Alk-Ph 邹鹏研究员科研成果|应用于真核细胞体系中的RNA邻近标记探针——Btn-An
