产品描述

Muscone, a flavouring ingredient, is an organic compound that is the primary contributor to the odor of musk and also a potent anti-inflammatory agent

靶点（IC50 & Targe）

MMP-9

P-gp (P-glycoprotein),

体外研究

Muscone induces CYP1A2 and CYP3A4 expression in liver cells in vitro and in vivo[1]. Muscone protects PC12 cells against glutamate-induced apoptosis by attenuating ROS generation and Ca2+ influx, via NR1 and CaMKII-depended ASK-1/JNK/p38 signaling pathways[2]. Muscone could promote bone marrow-derived mesenchymal stem cells (BMSCs) migration and proliferation[3].

体内研究

Muscone exhibits liver toxicity in Kunming mice at concentrations higher than 50 mg/kg[1]. It exerts potent neuroprotective activities on cerebral ischemia[2]. Muscone could accelerate the migration of BMSCs to the injured area in vivo[3].

细胞实验

Cell lines: PC12 cells

Concentrations: 0.1, 1, 10 μM

Incubation Time: 1 h

Method: PC12 cells are seeded in 96-well culture plates at an approximate density of 1×105 cells per well and maintained in a CO2 incubator at 37 ℃ overnight. At the end of indicated treatment, MTT is added to the medium and incubated for another 4 h at 37℃. After the removal of the culture medium, the insoluble dark blue formazan crystal is dissolved with 150 μL DMSO. The absorbance (A) is measured at 490 nm with a microplate reader. Cell viability is expressed as a percentage with the control group as 100%.

(Only for Reference)

参考文献

[1] Liu S, et al. Pharmacology. 2017, 99(5-6):205-215.

[2] Yu L, et al. Neurochem Int. 2014, 70:10-21.

[3] Pengfei Liu, et al. PLoS One. 2014, 9(5): e97123.