试剂仪器设备原料
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细胞培养基 >> 细胞株
BRL 3A大鼠肝细胞
BRL 3A大鼠肝细胞图片
产品介绍
目录号
Serial
GNR10
标识符
Identifier
CSTR:19375.09.3101RATGNR10
描述
Description
这个细胞株的无血清条件培养基是MSA的一个来源。 MSA多肽家族能够部分满足鸡胚成纤维细胞对血清的需求。
在本库通过支原体检测。
动物种别
Organism
大鼠
性别
Gender
***
组织来源
Tissue and Cell Type
形态
Morphology
***
培养基和添加剂
Complete Growth Medium and Culture Conditions
DMEM培养基(GIBCO by life technologies ,货号C11995500BT),90%;胎牛血清,10%。
气相:空气,95%;二氧化碳,5%。
温度:37摄氏度。
供应限制
Permits and Restrictions
A。仅供研究之用。
REFERENCENissley SP , et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146Coon HG , Weiss MC . A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097Dulak NC , Temin HM . A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141Dulak NC , Shing YW . Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. 90: 127-130, 1977. PubMed: 833209Schalch DS , et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol. Metab. 46: 664-671, 1978. PubMed: 755052
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