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细胞培养基 >> 细胞株
NIH/3T3小鼠胚胎成纤维细胞(小鼠,品系NIH/Swiss)
NIH/3T3小鼠胚胎成纤维细胞(小鼠,品系NIH/Swiss)图片
产品介绍
目录号
Serial
SCSP-515
标识符
Identifier
CSTR:19375.09.3101MOUSCSP515
描述
Description
NIH/3T3是从小鼠NIH/Swiss品系的胚胎中建立的成纤维细胞株。该细胞易于转染,对肉瘤病毒转化灶形成和白血病病毒增殖十分敏感。据报道,鼠痘病毒(ectromelia virus,ECTV)检测结果为阴性。
细胞质控结果:
1.支原体检测结果:每个批次均通过本库支原体检测,结果为阴性。
2.STR鉴定结果:
①该株细胞DNA进行小鼠细胞STR分型结果显示,扩增后图谱清晰,分型结果良好: 1-1: 10;1-2: 13, 17;2-1: 9;3-2: 14, 15;4-2: 19.3, 20.3;5-5: 14, 15;6-4: 15.3;6-7: 12;7-1: 29;8-1: 15;11-2: 15, 17;12-1: 20;13-1: 16.2;15-3: 20.3;17-2: 13, 14;18-3: 17, 19;19-2: 11, 12;X-1: 25。
②该细胞与ExPASy中NIH 3T3细胞的STR数据匹配率为100%。
③该株细胞确为小鼠细胞,没有人源细胞污染。
动物种别
Organism
小鼠,品系NIH/Swiss
性别
Gender

组织来源
Tissue and Cell Type
胚胎
形态
Morphology
成纤维细胞,贴壁生长
培养基和添加剂
Complete Growth Medium and Culture Conditions
NIH/3T3 完全培养液 配方(100 ml)
DMEM (Invitrogen, 11960-044)88 ml
新生牛血清 (Gibco)灭活后使用   10 ml
Glutamax (Invitrogen, 35050061)       1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070)  1 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
【注意事项:】
NIH/3T3细胞对于血清较为敏感,不能使用胎牛血清(FBS)培养该细胞。
生物安全等级:BSL-1
参考传代周期:2~3天
参考传代比例:1:4(备注:切勿使细胞生长过密,每周至少传代2次,保证细胞密度不超过80%。)
参考换液频率:2~3天
冻存液配方:完全培养液95%,DMSO 5%
供应限制
Permits and Restrictions
A。仅供研究之用。
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