产品介绍 目录号
Serial | SCSP-581 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP581 | 描述
Description | 人非小细胞肺癌细胞，分离自51岁黑人男性肺癌患者医疗前的肺癌组织，携带K-ras 12 突变，突变位于p53基因的246密码子位置， (ATC -> ATG, isoleucine -> methionine). 表达 C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, N-ras RNAs。每个批次均通过本库支原体检测，结果为阴性。在我库通过STR检测无误。STR鉴定结果为：Amelogenin: X; CSF1PO: 10; D13S317: 12; D16S539: 11; D5S818: 12,13; D7S820: 9,10; THO1: 6; TPOX: 8,9; vWA: 16,17 | 动物种别
Organism | 人 | 性别
Gender | 男性，51岁 | 组织来源
Tissue and Cell Type | 肺 | 形态
Morphology | 上皮样，贴壁 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人肺癌细胞NCI- H23完全培养液配方(100 ml)：
RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1 ml | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494Mitsudomi T, et al. p53 gene mutations in non-small-cell lung cancer cell lines and their correlation with the presence of ras mutations and clinical features. Oncogene 7: 171-180, 1992. PubMed: 1311061Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644Broers JL, et al. Spontaneous changes in intermediate filament protein expression patterns in lung cancer cell lines. J. Cell Sci. 91: 91-108, 1988. PubMed: 2473086Forsberg K, et al. Expression of functional PDGF beta receptors in a human large-cell lung- carcinoma cell line. Int. J. Cancer 53: 556-560, 1993. PubMed: 8382192Gazdar AF, et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40: 3502-3507, 1980. PubMed: 6108156NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.Lung Cancer 4: 155-161, 1988. |
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