细胞培养基 >> 细胞株
NCI-H1299人非小细胞肺癌细胞(2017年新引进)
产品介绍 目录号
Serial | SCSP-589 | 标识符
Identifier | CSTR:19375.09.3101HUMSCSP589 | 描述
Description | 人非小细胞肺癌细胞，这株细胞来源于一个淋巴结转移。细胞均一性的部分缺失p53蛋白，并缺少p53蛋白表达。细胞可以合成0.1 pmol/mg蛋白的NMB蛋白，而不合成促胃液释放肽(GRP)。每个批次均通过本库支原体检测，结果为阴性。在我库通过STR鉴定无误。STR结果为：Amelogenin:X; CSF1PO:12; D13S317:12; D16S539:12,13; D5S818:11; D7S820:10; THO1:6,9.3; TPOX:8; vWA: 16,17,18 | 动物种别
Organism | 人 | 性别
Gender | 男性，43岁 | 组织来源
Tissue and Cell Type | 肺；来源于淋巴结转移灶 | 形态
Morphology | 上皮样 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | 人肺癌细胞NCI-H1299完全培养液配方(100 ml)：
RPMI 1640 Medium (Invitrogen, 11875-093)88 ml
FBS (Gibco)10 ml
Glutamax(invitrogen 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Giaccone G, et al. Neuromedin B is present in lung cancer cell lines. Cancer Res. 52: 2732s-2736s, 1992. PubMed: 1563005NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.Lin DL, Chang C. p53 is a mediator for radiation-repressed human TR2 orphan receptor expression in MCF-7 cells, a new pathway from tumor suppressor to member of the steroid receptor superfamily. J. Biol. Chem. 271: 14649-14652, 1996. PubMed:8663350This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis from a patient who had received prior radiation therapy. |
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