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| 产品别名: | CCRF CEM人急性淋巴细胞白血病T淋巴细胞
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产品介绍 目录号
Serial | TCHu147 | 标识符
Identifier | CSTR:19375.09.3101HUMTCHu147 | 描述
Description | G.E. Foley 等人建立了类淋巴母细胞细胞株CCRF-CEM。 细胞是1964年11月从一位四岁白人女性急性淋巴细胞白血病患者的外周血白血球衣中得到。
在本库通过支原体检测。
在本库通过STR检测。 | 动物种别
Organism | 人 | 性别
Gender | 女 | 组织来源
Tissue and Cell Type | 白血病, 急性淋巴细胞白血病, 外周血, T淋巴母细胞 | 形态
Morphology | 淋巴母细胞 | 培养基和添加剂
Complete Growth Medium and Culture Conditions | RPMI 1640培养基(GIBCO by life technologies ,货号C11875500BT)，90%；优质胎牛血清，10%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 | 供应限制
Permits and Restrictions | A。仅供研究之用。 | | REFERENCE | Foley GE et al. Continous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522-529, 1965 Sandstrom PA and Buttke TM. Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl Acad. Sci. USA 90:4708-4712, 1993 PubMed: 93281648 Miranda L et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gpl60 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996 PubMed: 96353880 Adams RA. Formal discussion: the role of transplantation in the experimental investigation of human leukemia and lymphoma. Cancer Res. 27: 2479-2482, 1967 PubMed: 68134846 Uzman BG et ah Morphologic variations in human leukemic lymphoblasts (CCRF-CEM cells) after long-term culture and exposure to chemotherapeutic agents. A study with the electron microscope. Cancer 19:1725-I742, 1966 PubMed: 67(146473 Adams RA et al. Leukemia: serial transplantation of human leukemic lymphoblasts in the newborn Syrian halnster. Cancer Res. 27: 772-783. 1967 PubMed: 68122005 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2～a edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1 q88. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D.,(1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C. |
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