Methylated (ε-N) Lysine Antibody
Catalog # Product Description | ICP0501 The affinity purified rabbit polyclonal anti-methylated lysine antibody was developed using a technique unique to ImmuneChem. The methylated lysine antibodies are affinity purified using N-methyl (epsilon amino group) lysine on agarose as the affinity matrix. The antibodies could be utilized for detection, quantization and isolation of proteins with methylated lysine residues. |
| Western blot analysis of the methylated histone from melanoma cells with anti-methylated lysine anti-MeK (ICP0501). Histones were isolated with cold 0.1M HCl extraction followed by: A: Blot with 0.5 μg/mL of anti-MeK (ICP0501) in TBSt and blocked with synthetic methylated BSA 100 μg/mL B: Blot with 0.5 μg/mL of anti-MeK (ICP0501) in TBSt |
Species | Rabbit |
Formulation | 250 μg/mL in Tris-phosphate buffer, 50% glycerol |
Immunogen | Methylated KLH conjugates |
Purification | The antibody was immunoaffinity purified with epsilon N-methyl lysine on agarose. |
Specificity | The antibody recognizes proteins or peptides methylated on lysine residues (mono, di-methyllysine). There are no cross reactions with acetylated proteins. The cross reactivity with tri-methyl lysine has not been tested. |
Applications | ELISA; WB; IHC |
Scientific Description | MeK is a conserved post-translational modification and is an important biochemical process for many protein-protein interactions. It is found in many proteins, for example calmodulin, cytochrome C, chromosomal proteins, histones and non-histones as well as neural storage body proteins. It has been suggested that methylation of lysine plays an important role in gene silencing. [1-3] |
Storage & Stability | Store product at -20°C. Expiration date is one year from date of shipping if stored properly. |
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