Catalog# Product Description | ICP9806 Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source. |
|  Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806): Lane1-2: Kinase untreated Lane 3-6: kinase treated with phosphatase PP1G  Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates. A: Control B-D: Treatment with increased UV dose E: Phosphatase inhibitor treatment  High affinity anti-phospho-serine antibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin and α-casein) but not non-phospho proteins (ovalbumin, BSA, β-lactoglobulin, trypsinogen) |
| Species | Rabbit |
| Formulation |
250 μg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3 |
| Immunogen | Phospho-serine KLH and phosvitin mixture |
| Purification | Affinity purified with phospho-tyrosine on agarose |
| Specificity | The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine. |
| Tested | Human breast cancer tissue and mouse cell |
| Applications | ELISA (1:250); WB (1:250); IP (10 μg/mg crude protein); IHC (1:50) |
| Scientific Description | Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes. |
| Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |
Product Specific References | 1. Curr. Opin. Cell. Bio. 2001. 13(2): 131-138. doi:10.1016/S0955-0674(00)00189-7 2. Nat. Commun. 2016. 7: 11151. doi:10.1038/ncomms11151 3. Nature Communications. 2016. 7:11151: 1-15. doi:I: 10.1038/ncomms11151
4. Front Immunol. 2017; 8: 352. doi: 10.3389/fimmu.2017.00352. 5. Front Immunol. 2017; 8: 189. doi: 10.3389/fimmu.2017.00189. 6. JAHA. 2018; 7(1) doi: 10.1161/JAHA.117.007185 7. AJHG. 2018. 102(4): 649-657. doi: 10.1016/j.ajhg.2018.02.015 8. JAHA. 2018. 7(1). doi: 10.1161/JAHA.117.007185 9. Cellular Physiology and Biochemisty. 2018. 46(1). doi: 10.1159/000488418 10. Cell Reports. 2019. 28(3): 235-245. doi: 10.1016/j.celrep.2019.06.045
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